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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is noticed in many tumors, which ends up in chromosome instability, and thus may possibly add to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a critical part in endorsing mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but detailed nucleotide sequence info isn't still readily available.
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Blocking peptides are peptides that bind especially to the goal antibody and block antibody binding. These peptide usually includes the epitope identified from the antibody.
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This guide outlines additional attainable optimization of mobile and immunoassay parameters to be certain the best possible results are...
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This manual outlines even further doable optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...
Antibodies bound to the blocking peptide not bind for the epitope within the target protein. By evaluating the staining with the blocked antibody compared to the antibody by yourself, you can see which staining is specific.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose associates are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins show unique expression and degradation patterns which add to your temporal coordination of each mitotic celebration. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates on the G1-S period boundary and is particularly degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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